Elsa and Julia heroically dug through decades of primary literature on mammalian neuronal mRNA transport to generate a concise overview on how mRNAs are possibly coupled to motor proteins: https://doi.org/10.3389/fnint.2021.692948.
We also connected minimal mRNA-transport subnetworks with information about motor selectivity for specific neurites (axon/dendrites) to develop ideas which motors and adaptors might be required for different transport steps:
New preprint from our lab! To probe the potential for complex formation among RBPs, we assembled a library of 978 mammalian RBPs and used rec-Y2H screening to detect direct interactions between RBPs, sampling > 600 K interactions. We discovered 1994 new interactions and demonstrate that our screen detects interactions between RBPs that bind RNAs adjacently. We further find that the mRNA binding region preferences of an RBP can deviate depending on its adjacently binding interaction partner. Finally, we reveal novel RBP interaction networks among major RNA processing steps and show that RBP mutations observed in cancer rewire spliceosomal interaction networks.
Oh yes – and of course we found a number of interesting new links between mRNA binding proteins and kinesins & dynein.
We built a first minimal axonal mRNA transport system: Reconstituted APC-kinesin-2 complexes selectively transport G-motif containing mRNAs with mostly two mRNAs per complex. Yellow: beta-actin mRNA, cyan: mutated beta-actin mRNA. https://advances.sciencemag.org/content/6/11/eaaz1588
Elsa (re-) joined our lab as PhD student and she managed to obtain a LaCaixa INPhINIT PhD fellowship. Congratulations Elsa and welcome back!
The first reconstitution of a mammalian mRNA transport system done by Sebastian Bauman is online:
In the movie below we see beta2B-tubulin RNA (red) transported by a reconstituted APC-kinesin-2 complex on microtubules (grey). The lower panel shows single-particle tracking of the transported RNAs (circle color around spots: total spot intensity). We used intensity measurements to show that one kinesin transports dimeric APC which binds one RNA per APC monomer.
Stay tuned for news on the first reconstituted axonal mRNA transport system
We are looking for PhD students to work with systems and biochemical approaches to understand how RBPs work together and how neurons can generate their mRNA distribution patterns. Please check out the Jobs-Section. And apply through CRG’s PhD Fellowship programme.
Our new paper describing two new matrix screening techniques is out !
Julia Grawenhoff got a Severo-Ochoa FPI PhD fellowship to join our lab in spring 2018. Welcome Julia !
Sebastian Baumann got a Juan de la Cierva postdoc fellowship to continue his promising work in our lab. Congratulations Sebastian!
Artem joined the lab. He won a LaCaixa-PhD fellowship and he will focus on developing ex-vivo tools to understand mRNP dynamics and function. Welcome, Artem !
03 / 2016
Our lab won an HFSP Young Investigator Grant together with the labs of Ivo Telley, Martin Loose and Timothy Saunders.
11 / 2015
Andrea joins the lab on a Severo Ochoa PhD fellowship – Welcome Andrea
10 / 2015
We were awarded the Europa Excelencia grant of the Spanish MINECO
06 / 2015
Sebastian Baumann got a Marie-Curie fellowship – Congratulations !
04 / 2015
Lorenzo joined the lab to carry out a 6 month Leonardo Student project – Welcome !!!
01 / 2015
SebastianB joined the lab as a Postdoc – Welcome Sebastian !
Bogumilla started her PhD in our lab, supported by an LaCaixa fellowship, welcome !